Dr. Alok Krishna Sinha
    Staff Scientist IV
    Ph.D: National Botanical Research Institute, Lucknow
    Postdoctoral Fellow: University of Regensburg (AvH fellow) and University of Wuerzburg Germany
    Tel: 91-11-26741612,14,17 Ext. - 188
    Direct - 26735188
    Fax: 91-11-26741658
    E-mail: alok_sinha@nipgr.res.in, alokksinha@yahoo.com
 Research Interest
1. Dissecting mitogen activated protein kinase (MAPK) cascade in rice
Our group is interested in understanding the complexity of signals being transduced through mitogen activated protein kinase (MAPK) cascade in rice. The MAPK cascade is evolutionarily conserved among the eukaryotes and typically consists of three types of protein kinases, MAPK, MAPK kinase (MAPKK), and MAPKK kinase (MAPKKK). By responding to external stimuli, the MAPK cascade plays a critical role in gene expression, metabolism, cell death, proliferation, and differentiation in animals and yeast. In plants, the MAPK cascade is also involved in various biotic and abiotic stress responses, hormone responses, cell division, and developmental processes. Complete sequencing of the Oryza sativa genome has revealed that the MAPK family consists of 8 MAPKK and 16 MAPK genes. By using a combination of molecular and biochemical techniques along with genomics and proteomic approaches we are trying to identify the function of specific MAPKs in rice. We have a pool of rice MAPKs, MAPKKs and MAPKKKs and with the help of protein-protein interactions, coimmuno-precipitation and plant transformation either for the over expression or silencing by RNAi/Artificial miRNA strategy, we are trying to dissect the MAPK cascade being stimulated by different stresses.
 Group Members
 Detailed Research Publications
 Selected Research Publications
Jaggi M, Kumar S and Sinha AK (2011). Overexpression of an apoplastic peroxidase gene CrPrx in transgenic hairy root lines of Catharanthus roseus. Applied Microbiology and Biotechnology (In press) DOI: 10.1007/s00253-011-3131-8.
Kumar S, Jaggi M, Taneja J and Sinha AK (2011). Cloning and characterization of two new Class III peroxidase genes from Catharanthus roseus. Plant Physiology and Biochemistry (In Press) DOI: 10.1016/j.plaphy.2011.01.012.
Kumar K, Rao KP, Biswas D and Sinha AK (2011). Rice WNK1 is regulated by abiotic stress and involved in internal circadian rhythm. Plant Signaling and Behavior Volume 6, Issue 3 (In press).
Sinha AK, Jaggi M, Raghuram B, and Tuteja N (2011). Mitogen activate dprotein kinase signaling in plant under abiotic stress. Plant Signaling and Behavior Volume 6, Issue 2 (In press).
Bhardawaj D, Sheikh AH, Sinha AK and Tuteja N (2011). Stress induced beta subunit of heterotrimeric G-proteins from Pisum sativum interacts with mitogen activated protein kinase. Plant Signaling and Behavior Volume 6, Issue 2 (In press)
Rao KP, Vani G, Kumar K, Wankhede DP, Misra M, Gupta M and Sinha AK (2011). Arsenic stress activates MAP kinase in rice roots and leaves. Archieves of Biochemistry and Biophysics 506: 73-82.
Hyun TK, Kumar K, Rao KP, Sinha AK, Roitsch T (2011) Role of a-tocopherol in cellular signaling: a-tocopherol inhibits stress-induced mitogen activated protein kinase activation. Plant Biotechnology Reports, 5: 19-25.
Bonfig KB, Gabler A, Simon UK, Luschin-Ebengreuth N, Hatz M, Berger S, Muhammad N, Zeier J, Sinha AK, Roitsch T (2010) Post-translational depression of invertase activity in source leaves via down regulation of invertase inhibitor expression is part of the plant defense response. Molecular Plant, 3: 1037-1048.
Taneja J, Jaggi M, Wankhede DP, and Sinha AK (2010) Effect of loss of T-DNA genes on MIA biosynthetic pathway gene regulation and alkaloid accumulation in Catharanthus roseus hairy roots. Plant Cell Rep. 29(10): 1119-1129.
Rao KP, Richa T, Kumar K, Raghuram B and Sinha AK (2010). In silico analysis reveals 75 members of mitogen activated protein kinase kinase kinase gene family in rice. DNA Research, 17(3): 139-53.
Rao KP, Vani G, Kumar K and Sinha AK (2009) Rhythmic expression of Mitogen Activated Protein Kinase Activity in Rice. Molecules and Cells 28: 417-422.
Hyun TK, Hoffmann A, Sinha AK, Roitsch T (2009) Tomato mitogen activated protein kinases regulate the expression of extracellular invertase Lin6 in response to stress related stimuli. Functional Plant Biology 36: 1088-97.
Gupta M, Sharma P, Sarin NB, and Sinha AK (2009). Differential responses of arsenic stress in two varieties of Brassica juncea. Chemosphere 74: 1201-1208.
Kumar K, Rao KP, Sharma P and Sinha AK (2008). Differential regulation of rice mitogen activated protein kinase kinase (MKK) by abiotic stress. Plant Physiology and Biochemistry 46: 891-897.
Hofmann MG, Sinha AK, Proels RK and Roitsch T (2008) Cloning and characterization of a novel LpWRKY1 transcription factor in tomato. Plant Physiology and Biochemistry 46(5-6): 533-40.
Berger S, Sinha AK and Roitsch T (2007). Plant physiology meets phytopathology: relations between plant primary metabolism and plant-pathogen-interactions. Journal of Experimental Botany 58: 4019-2026.
Kumar S, Dutta A, Sinha AK and Sen J (2007). Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus. FEBS Journal 274: 1290-1303.
Thoma I, Loeffler C, Sinha AK, Gupta M, Krischke M, Steffan B, Roitsch T and Mueller MJ (2003) Cyclopentenone isoprostanes induced by reactive oxygen species trigger defense gene activation and phytoalexin accumulation in plants. The Plant Journal 34: 363-375. (Cover article)
Roitsch T, Balibrea ME, Hofmann M, Proels R and Sinha AK (2003) Extracellular invertase: key metabolic enzyme and PR protein. Journal of Experimental Botany 54, 382: 513-24.
Link V, Sinha AK, Vasistha P, Hofmann MG, Proels RK, Ehness R and Roitsch T (2002). Activation of a MAPK by heat in tomato cell suspension cultures. FEBS Letter 531, 2: 179-183.
Sinha AK, Hofmann, M, Romer U, Kockenberger W, Elling L and Roitsch T (2002). Metabolizable and non-metabolizable sugars activate different signal transduction pathways in tomato. Plant Physiology 128: 1480-1489.
Link V, Hofmann M, Sinha AK, Ehness R, Strnad M and Roitsch T (2002). Biochemical evidence for the activation of distinct subsets of mitogen-activated protein Kinase by voltage and defence related stimuli. Plant Physiology 128: 271-281.