National Institute of Plant Genome Research
 
    Dr. Ashverya Laxmi
    Staff Scientist III
    Tel: 91-11-26741612,14,17 Ext. - 180
    Direct - 26735180
    Fax: 91-11-26741658
    E-mail: ashverya_laxmi@nipgr.ac.in, laxmiashverya@rediffmail.com
 Career
September 2006 - Staff Scientist II, National Institute for Plant Genome Research
2005-2006 Post Doctoral Fellow, Samuel Robert Noble Foundation, Oklahoma, USA
2004-2005 Research Scientist, Centre of Plant Molecular Biology, University of Delhi
2003-2004 Post Doctoral Fellow, Ohio State University, Ohio, USA
2002-2003 SRF, Department of Plant Molecular Biology, University of Delhi
1997-2002 Ph.D. in Plant Molecular Biology from the Department of Plant Molecular Biology, University of Delhi
 Research Interests
Plants being sessile have developed a sophisticated machinery to perceive and respond to continuously changing environmental conditions. The exogenous signal is perceived either via membrane bound receptors, cytosolic receptors or receptors associated with different intracellular organelles. After the perception of the signal, a cascade of events starts which transduce it to the plant gene machinery to modulate gene expression and in turn plant development. These signalling cascades may involve different events such as changed ca2+ ion homeostasis, phosphorylation/dephsphorylation reactions, protein-protein interactions and so on. Earlier, this whole cascade was believed to be linear i.e. one signal evoking only one signalling pathway but with the plethora of reports pouring in, it is becoming increasingly clear that all these signalling pathways interact with each other and rather than being linear they are intermingled and are part of a web. Broadly speaking, one stimulus rather than evoking one signalling event can interact with several other signalling pathways so as to optimally modulate plant growth and development. These signalling interactions can happen at any level i.e. sharing of signalling components by multiple pathways, or convergence of signalling pathways at the level of transcription factors or further downstream at the promoter of the affected gene itself. There are several reports existing in plants, where light, hormone, sugar and stress signalling pathways have been shown to be interacting. Being fascinated by this complex interaction existing in signalling world, I would like to focus my research on understanding the molecular mechanism underlying these signalling interactions. In the past, using Arabidopsis as a model system, I screened M2 population of fast neutron bombarded mutants and isolated several constitutive photomorphogenic mutants which were simultaneously defective in sugar and hormone response pathways. As of now, my interest would be to clone the mutant genes of these mutants defective in multiple signal transduction pathways. They will not only define the molecular nodes or switches underlying signalling interaction but will also provide a further insight in to the molecular mechanism of signalling interaction. Another approach to elucidate this phenomenon would be to individually study the nature of interaction between already known signalling pathways using physiological, cell biological, genetical and molecular approaches. For that matter, I am presently focussing on elucidating sugar/auxin and sugar/brassinosteroid interactions, individually. The identified gene(s) may later be employed improving agronomically important traits in crop plants.
 Selected Publications
Kushwah S, Laxmi A (2013) The interaction between glucose and cytokinin signal transduction pathway in Arabidopsis thaliana. Plant Cell Environ. doi: 10.1111/pce.12149. [Epub ahead of print]
Gupta A, Singh M, Jones AM, Laxmi A (2012) Hypocotyl directional growth in Arabidopsis: a complex trait. Plant Physiology 159: 1463-1476
Kushwah S, Jones AM, Laxmi A (2011) Cytokinin-induced root growth involves actin filament reorganization. Plant Signaling Behavior 6 (11): 1848-1850
Kushwah S, Jones AM, Laxmi A (2011) Cytokinin interplay with ethylene, auxin and glucose signaling controls Arabidopsis seedling root directional growth. Plant Physiology 156: 1851-1866
Murray JD, Muni RR, Torres-Jerez I, Tang Y, Allen S, Andriankaia M, Li G, Laxmi A, Cheng X, Wen J, Vaughan D, Schultze M, Sun J, Chamentier M, Oldroyd D, Tadege M, Ratet P, Mysore KS, Chen R, Udvardi MK (2011) Vapyrin, a gene essential for intracellular progression of arbuscular mycorrhizal symbiosis, is also essential for infection by rhizobia in the nodule symbiosis of Medicago truncatula. Plant Journal 65(2): 244-252.
Gupta A, Singh M, Mishra BS, Kushwah S, Laxmi A (2009) Role of glucose in spatial distribution of auxin regulated genes. Plant Signal Behav. 4(9): 862-863.
Tripathi V, Syed N, Laxmi A, Chattopadhyay D (2009) Role of CIPK6 in root growth and auxin transport. Plant Signal Behav. 4 (7): 663 - 665
Mishra BS, Singh M, Aggrawal P and Laxmi A (2009) Glucose and auxin signaling interaction in controlling Arabidopsis thaliana seedlings root growth and development. PLoS ONE 4: e4502.
Tripathi V, Parasuraman B, Laxmi A and Chattopadhyay D (2009) CIPK6, a CBL-interacting protein kinase is required for development and salt tolerance in plant. Plant Journal 58(5): 778-90.
Laxmi A, Pan J, Morsy M and Chen R (2008) Light plays an essential role in intracellular distribution of auxin efflux carrier PIN2 in Arabidopsis thaliana. PLoS ONE 3(1): e1510.
Laxmi A, Paul LK, Chaudhuri AR, Peters JL and Khurana JP (2006) Arabidopsis cytokinin resistant mutant, cnr1, displays altered auxin response and sugar sensitivity. Plant Molecular Biology, 62: 409-425.
Price J, Laxmi A, St. Martin SK, and Jang JC (2004) Global Transcription Profiling Reveals Multiple Sugar Signalling Mechanisms in Arabidopsis. Plant Cell, 16: 2128-2150.
Laxmi A, Paul LK, Peters JL and Khurana JP (2004) Arabidopsis constitutive photomorphogenic mutant bls1, displays altered brassinosteroid response and sugar sensitivity. Plant Molecular Biology, 56: 185-201.
Khurana JP, Dasgupta U, Laxmi A, Kumar D and Paul LK (2004) Light Control of Plant Development by Phytochromes: A Perspective. Proc. Indian Natl. Sci. Acad., B70: 379-411.
Khurana JP, Tyagi AK, Khurana P, Kochhar A, Jain PK, Raychaudhuri A, Chawla R, Bharti AK, Laxmi A and Dasgupta U (1999) Molecular genetic analysis of constitutively photomorphogenic mutants of Arabidopsis In: S.K. Sopory, S.C. Maheshwari and R. Oelmuller, (s), Signal Transduction in Plants - Current Advances, pp. 25-37. Kluwer Academic Publishers, New York.