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 | Dr. Debasis Chattopadhyay
Staff Scientist V
Ph.D. - Calcutta University
Pos-Doctoral Fellow - The Cleveland Clinic Foundation, Ohio
Tel: 91-11-26741612,14,17 Ext. - 189
Direct - 26735189
Fax: 91-11-26741658
E-mail: debasis_chattopadhyay@nipgr.res.in chattod@yahoo.co.in |
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 Research Interest |
| Our program focuses on three major areas of research, namely, improvement of tolerance to abiotic stresses (i.e. drought and salt) in higher plants by improving root traits, and developing resistance in Soybean against Geminivirus infection and genome sequencing. |
| Abiotic Stress Tolerance |
| Root cells are first to respond to change in soil conditions. Continuous change in root system architecture is the vital part of the developmental plasticity that allows the plant to adjust in altered environment. Phytohormone auxin is the most important regulator of all the stages of root development. Recently, an unprecedented role of stress-related phytohormone Abscisic acid in regulation of lateral root formation is emerging. We are investigating the nodal point of interplay between auxin and abscisic acid. We primarily isolate genes from Chickpea, which is the third most important legume crop and adapts well in water-limited environment. |
| We have shown overexpression of a Chickpea kinase CaPKS and two transcription factors CAP2 and CaZF also increases lateral root formation in Tobacco and Arabidopsis by altering auxin signaling. Our long term goals are to use genetic engineering to increase root biomass production and tolerance to abiotic stresses (salinity, drought) of important crops. A more immediate goal is to understand the cellular mechanisms involved in the above phenotypes. |
| MYMV-Soybean interaction |
| Geminivirus infection causes a considerable loss in Soybean productivity in India. We focus our attention on Mungbean Yellow Mosaic India Virus (MYMIV) that infects fourteen legume crops along with Soybean. MYMIV infection is spread by White Fly, which acts as the vector. Screening of population derived from crossing with naturally resistant wild Soybean is dependent on White fly infection, which is difficult to control. We have constructed a MYMIV infectious clone that is 100% efficient in producing symptoms through agroinoculation. We are currently investigating the mechanism of natural resistance in some of the Soybean varieties. |
| Tomato Genome Sequencing |
| Our laboratory is a part of the Indian Initiative on Tomato Genome Sequencing, a part of the International Solanaceae Genome Sequencing Network. We are sequencing a part of the Chromosome 5 of tomato. We are also involved in sequencing the whole genome of a cultivated and a wild variety of tomato using next generation sequencing approach. |
| Publications |
 | Tripathi V, Parasuraman B, Laxmi A and Chattopadhyay D (2009) CIPK6, a CBL-interacting protein kinase is required for development and salt tolerance in plant. Plant Journal 58:778-790 |
| Shukla RK, Raha S, Tripathi V and Chattopadhyay D (2006) Expression of CAP2, an AP2-family transcription factor from Chickpea enhances growth and tolerance to dehydration and salt tress in transgenic tobacco. Plant Physiology 142: 113-123. |
| Boominathan P, Shukla RK, Kumar A, Manna D, Negi D, Verma PK and Chattopadhyay D (2004) Long term transcript accumulation during the development of dehydration adaptation in Cicer arietinum L. Plant Physiology 135(3):1608-1620. |
| Jain D, Roy N and Chattopadhyay D (2009) CaZF, a plant transcription factor functions independent of Hog1p and Calcineurin in Saccharomyces cerevisiae to provide osmotolerance. PLoS ONE 4(4):e5154 |
| Shukla RK, Vripathi V, Jain D, Yadav RK and Chattopadhyay D (2009) CAP2 enhances germination of transgenic tobacco seeds at high temperature and promotes heat stress tolerance in yeast. FEBS Journal (doi:10.1111/j.1742-4658.2009.07219.x) |
| Yadav RK, Shukla RK and Chattopadhyay D (2009) Soybean cultivar resistant to Mungbean Yellow Mosaic India Virus infection induces viral RNA degradation earlier than the susceptible cultivar. Virus Research 144: 89-95 (doi:10.1016/j.virusres.2009.04.011). |
| Tripathi V, Syed N, Laxmi A and Chattopadhyay D (2009) Role of CIPK6 in root growth and auxin transport. Plant Signaling and Behavior 4(7):663-665. |
| Shridhar S, Chattopadhyay D and Yadav G (2009) PLecDom: A Program for identification and Analysis of Plant Lectin Domains. Nucleic Acid Research Jul 1; 37: W452-8. Epub 2009 May 27 |
| Kaur H, Shukla RK, Yadav G, Chattopadhyay D and Majee M (2008) Two divergent genes encoding L-myo-inositol 1-phosphate synthase1 (CaMIPS1) and 2 (CaMIPS2) are differentially expressed in chickpea. Plant Cell Environ 31:1701-1716 |
| Yadav V, Kundu S, Chattopadhyay D, Negi P, Wei N, Deng XW and Chattopadhyay S (2002) Light regulated modulation of Z- box containing promoters by photoreceptors and downstream regulatory components, COP1 and HY5 in Arabidopsis. Plant Journal 31(6): 731-753. |
| The Tomato Genome Sequencing Consortium (2009) A snapshot of the emerging tomato genome sequence. The Plant genome 2(1): 78-92 |
| Mueller LA, Tanksley SD, Chattopadhyay D and Zamir D. (SOL genome consortium). (2005) The Tomato Sequencing Project, the first cornerstone of the International Solanaceae Project (SOL). Comparative and Functional Genomics 6(3): 153-158. |
| Nayak S, Balaji J, Upadhyay HD, Hash CT, KaviKishore PB, Chattopadhyay D, Rodrigues LM, Blair MW, Baum M, McNally K, This D, Hosington D and Varshney R (2009) Isolation and sequence analysis of DREB2A homologues in three cereal and two legume species. Plant Science (in press). |
| Chattopadhyay D, Ghosh MK, Mal A, and Harter ML (2001) Inactivation of p21 by E1A Leads to the Induction of Apoptosis in DNA-Damaged Cells. J. Virol. 5: 9844-9856. |
| Basak S, Raha T, Chattopadhyay D, Majumder A, Shaila MS and Chattopadhyay DJ (2003) Leader RNA Binding Ability of Chandipura Virus P protein is Regulated by its Phosphorylation Status: A Possible Role in Genome Transcription-Replication Switch. Virology 307: 372-385. |
| Mal A, Chattopadhyay D, Ghosh M, Poon RYC, Hunter T and Harter ML (2000) p21 and Retinoblastoma protein control the absence of DNA replication in terminally differentiated muscle cells. Jour. of Cell Biology 149(2): 281-292. |
| Raha T, Chattopadhyay D and Chattopadhyay D (2000) N-terminal region of P protein of Chandipura virus is responsible for phosphorylation-mediated homodimerization. Protein Engineering 13(6): 437-444. |
| Raha T, Chattopadhyay D, Chattopadhyay D and Roy S (1999). A phosphorylation induced major structural change in the N-terminal domain of the P protein of Chandipura virus. Biochemistry 38(7): 2110-2116. |
| Chattopadhyay D, Raha T, Chattopadhyay D (1997) PCR mutagenesis:Treatment of megaprimer with mung bean nuclease improves yield. BioTechniques 22: 1054-1056. |
| Chattopadhyay D, Raha T, Chattopadhyay D (1997) Single Serine phosphorylation within acidic domain of Chandipura virus P protein regulates the transcription in vitro. Virology 239: 11-19. |
| Chattopadhyay D and Chattopadhyay D (1994). Cloning of Chandipura virus phosphoprotein encoding gene and its expression in E.coli. Cell. Mol. Biol. Res. 40: 693-698 |
| Biswas S, Gupta MK, Chattopadhyay D and Mukhopadhyay CK (2007). Insulin induced activation of Hypoxia inducible factor-1 requires generation of reactive oxygen species by NADPH oxidase. Am. J. Physiol: Heart Circ. Physiol. 292: 758-766 |
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| Patent Filed |
| "Chimeric Construct of Mungbean Yellow Mosaic India Virus (MYMIV) and its uses thereof" - IPA-2571/DEL/2007 and PCT/IN/2008/000819. |
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