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 | Dr. Niranjan Chakraborty
FNASc., Staff Scientist VI
Ph. D : Jawaharlal Nehru University, New Delhi
Tel: 91-11-26735178
Fax: 91-11-26741658
E-mail: nchakraborty@nipgr.res.in, nchakraborty@hotmail.com |
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 Research Area |
| The research program focuses on elucidation of the molecular mechanism(s) underlying plant response to abiotic stress, using a combination of physiochemical, proteomics, and genomics approaches. This would guide the identification of candidate genes and gene-products and form the basis for strategies for their targeted manipulation towards improved adaptation to such stress. Another research interest of my laboratory is the improvement of the nutritional quality of crop. |
| Stress Genomics |
| It is increasingly apparent that plants, being sessile, have evolved many adaptations to counteract various environmental stresses. Our aim is to determine the molecular circuitry that operates in the cellular response to such stresses. The alteration of protein synthesis or degradation is one of the fundamental metabolic processes that may influence stress tolerance. We are developing high-throughput proteomics approaches to study stress perception, signal transduction and metabolic responses of plants to various environmental stresses. We are focusing on subcellular proteomics and using a number of proteome mining tools in order to understand the role of protein modifications and/or their differential expression under stress conditions. The comparative proteomic analysis would enable the identification of key regulatory proteins thereby providing valuable insight into the diverse, complex, and dynamic network operating under stress. The major focus would be on identifying the dynamics of stress-responsive proteins (SRPs) to discover the regulatory genes that control stress tolerance and their manipulation in transgenic plants. |
| Nutritional Genomics |
| The other area of our interest is to improve the nutritional quality of crop plants by introducing quality traits. Keeping in view the importance of dietary protein and micronutrients, and the fact that plants are their major source, development of value-added transgenic crops is of our primary interest. During recent years, we have been successful in increasing the nutritional quality of potato. Currently, we are using few other novel genes to improve the bioavailability of microelements in edible crops. We have developed efficient technologies for genetic transformation of several of the carbohydrate-rich, non-conventional staple crops as a step towards tailoring their nutritional status. |
| Selected Publications |
 | Chattopadhyay A, Subba P, Pandey A, Bhushan D, Kumar R, Datta A, Chakraborty S and Chakraborty N (2011) Analysis of the grasspea proteome and identification of stress-responsive proteins upon exposure to high salinity, low temperature and abscisic acid treatment. Phytochemistry 72: 1293-1307. |
| Bhushan D, Jaiswal DK, Ray D, Basu D, Datta A, Chakraborty S and Chakraborty N (2011) Dehydration-responsive reversible and irreversible changes in the extracellular matrix: comparative proteomics of chickpea genotypes with contrasting tolerance. J. Proteome Res. 10: 2027-2046. |
| Ghosh S, Meli VS, Kumar A, Thakur A, Chakraborty N, Chakraborty S and Datta A (2011) The N-glycan processing enzymes α-mannosidase and β-D-N-acetylhexosaminidase are involved in ripening-associated softening in the non-climacteric fruits of capsicum. J. Exp. Bot. 62: 571-582. |
| Chakraborty S, Chakraborty N, Agrawal L, Ghosh S, Narula K, Shekhar S, Naik PS, Pande PC, Chakraborti SK and Datta A (2010) Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber. Proc. Natl. Acad. Sci. USA 107: 17533-17538. |
| Agrawal GK, Bourguignon J, Rolland N, Ephritikhine G, Ferro M, Jaquinod M, Alexiou KG, Chardot T, Chakraborty N, Jolivet P, Doonan JH and Rakwal R (2010) Plant organelle proteomics: Collaborating for optimal cell function. Mass Spectrom. Rev. PMID 21038434. |
| Pandey A, Rajamani U, Verma J, Subba P, Chakraborty N, Datta A, Chakraborty S and Chakraborty N (2010) Identification of extracellular matrix proteins of rice (Oryza sativa L.) involved in dehydration-responsive network: a proteomic approach. J. Proteome Res. (in press). |
| Meli VS, Ghosh S, Prabha TN, Chakraborty N, Chakraborty S and Datta A (2010) Enhancement of fruit shelf life by suppressing N-glycan processing enzymes. Proc. Natl. Acad. Sci. USA 107: 2413-2418. |
| Ashraf N, Ghai D, Barman P, Basu S, Nagaraju G, Mondol MK, Chakraborty N, Datta A and Chakraborty S (2009) Comparative analyses of genotype-dependent expressed sequence tags and stress-responsive transcriptome of chickpea wilt illustrates predicted and unexpected genes and novel regulators of plant immunity. BMC Genomics 10: 415. |
| Chaudhary MK, Basu D, Datta A, Chakraborty N and Chakraborty S (2009) Dehydration-responsive nuclear proteome of rice (Oryza sativa L.) illustrates protein network, novel regulators of cellular adaptation and evolutionary perspective. Mol. Cell. Proteomics 8: 1579-1598. |
| Agrawal L, Chakraborty S, Jaiswal DK, Gupta S, Datta A and Chakraborty N (2008) Comparative proteomics of tuber induction, development and maturation reveal the complexity of tuberization process in potato (Solanum tuberosum L.) J. Proteome Res. 7: 3803-3817. |
| Pandey A, Chakraborty S, Datta A and Chakraborty N (2008) Proteomics approach to identify dehydration responsive nuclear proteins from chickpea (Cicer arietinum L.). Mol. Cell. Prot. 7: 88-107. |
| Bhushan D, Pandey A, Choudhary MK, Datta A, Chakraborty S and Chakraborty N (2007) Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress. Mol. Cell. Prot. 6: 1868 -1884. |
| Pandey A, Choudhary MK, Bhushan D, Chattopadhyay A, Chakraborty S, Datta A and Chakraborty N (2006) The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins. J. Proteome Res. 5: 3301-3311. |
| Chakraborty N, Ohta MO and Zhu JK (2006) Recognition of a PP2C interaction motif in several plant protein kinases. Methods in Molecular Biology. 365: 287-298. |
| Bhushan D, Pandey A, Chattopadhyay A, Choudhary MK, Chakraborty S, Datta A and Chakraborty N (2006) Extracellular matrix proteome of chickpea (Cicer arietinum) illustrates pathway abundance, novel protein functions and evolutionary perspect. J. Proteome Res. 5: 1711-1720. |
| Chakraborty S, Chakraborty N, Jain D, Salunke DM and Datta A (2002). Active site geometry of oxalate decarboxylase from Flammulina velutipes: Role of histidine coordinated manganese in substrate recognition. Protein Sci. 11: 2138-2147. |
| Chakraborty S, Sarmah B, Chakraborty N and Datta A (2002) Premature termination of RNA polymerase II mediated transcription of a seed protein gene in Schizosaccharomyces pombe. Nuclei Acids. Res. 30: 2940-2949. |
| Sarmah B, Chakraborty N, Chakraborty S and Datta A (2002) Plant pre-mRNA splicing in fission yeast, Schizosaccharomyces pombe. Biochem. Biophy. Res. Commn. 293: 1209-1216. |
| Chakarborty S, Chakarborty N and Datta A (2000). Increased nutritive value of transgenic potato by exspressing a nonallergenic seed albumin gene from Amaranthus hypochondriacus. Proc. Natl. Acad. Sci. USA. 97: 3724-3729. |
| Chakarborty N and Tripathy BC (1992) Involvement of singlet oxygen in 5-aminolevulinic acid induced photodynamic damage of cucumber (Cucumis sativus L.) chloroplasts. Plant Physiol. 98: 7-11. |
| Tripathy BC and Chakraborty N (1991) 5- aminolevulinic acid induced photodynamic damage to the photosynthetic electron transport chain of cucumber (Cucumis sativus L.) cothledons. Plant Physiol. 96: 761-767. |
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