 |
|
|
|
|
|
| |
|
 | |  |
|
 | Dr. Pinky Agarwal
Staff Scientist II
M. Sc. and Ph. D. (Plant Molecular Biology-South Campus Delhi University)
Tel: 91-11-26735227
Fax: 91-11-26741658
E-mail: pinky.agarwal@nipgr.res.in |
|
 | |  |
 Research Area |
 | Molecular Biology, Genomics, Transcriptomics |
|
| Research Interest |
| Rice is the staple food of more than 50% of the world's population. The edible part is the seed or grain. Based on the morphological changes occurring during rice seed development, it can be classified into five stages. Microarray was performed for these stages and compared with vegetative controls. The microarray data was validated by Q-PCR and by promoter-reporter constructs. Detailed analysis of microarray data showed that transcription factors form one of the major classes of differentially expressed genes. With this in view, the C2H2 zinc finger encoding gene family members were identified from the rice genome. In silico analysis was performed for 189 family members at the gene and protein levels and their phylogeny was also studied. Transgenic rice plants were also raised for genes showing high expression in the seed. Ongoing work includes delving deeper into the molecular biology of rice seed development and establishment of operating networks. |
| Selected Publications |
| Agarwal P, Kapoor S, Tyagi AK (2011). Transcription factors regulating the progression of monocot and dicot seed development. BioEssays 33: 189-202. |
| Agarwal P, Arora R, Ray S, Singh AK, Singh V P, Takatsuji H, Kapoor S, Tyagi AK (2007). Genome-wide identification of C2H2 zinc-finger gene family in rice and their phylogeny and expression analysis. Plant Mol. Biol. 65: 467-485. |
| Ray S, Agarwal P, Arora R, Kapoor S, Tyagi AK (2007). Expression analysis of calcium-dependent protein kinase gene family during reproductive development and abiotic stress conditions in rice (Oryza sativa L. ssp. indica). Mol. Genet. Genomics 278: 493-505. |
| Arora R, Agarwal P, Ray S, Singh AK, Singh VP, Tyagi AK, Kapoor S (2007). MADS-box gene family in rice: Genome wide identification, organization and expression profiling during reproductive development and stress. BMC Genomics 8: 242. |
| Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007). F-box proteins in rice: Genome-wide analysis, classification, spatial and temporal gene expression during panicle and seed development, and regulation by light and abiotic stress. Plant Physiol. 143: 1467-1483. |
| Ray S, Dansana PK, Giri J, Deveshwar P, Arora R, Agarwal P, Khurana JP, Kapoor S, Tyagi AK (2011). Comparative analysis of water-deficit stress responsive genes vis-a-vis genes expressing differentially on exposure to cold and salt stress and during reproductive development in rice. Funct Integr Genomics 11: 157-78. |
|
| Book Chapters |
| Tyagi AK, Khurana JP, Khurana P, Kapoor S, Singh VP, Singh AK, Thakur JK, Gupta V, Anand S, Vij S, Jain M, Ray S, Agarwal P, Arora R, Sharma P, Mukerjee S, Nijhawan A, Giri J, Khurana R (2007). Expression and functional analysis of rice genes involved in reproductive development and stress response. Rice Genetics V, 301-334, IRRI, Philippines. |
| Kapoor S, Khurana R, Baranwal V, Agarwal P, Ray S, and Tyagi AK (2011). Genome-wide Strategies for Genetic Enhancement of Rice. Proceedings of National Symposium on Genomics and Crop Improvement 2011 (In Press), Hyderabad, India. |
|
| Patent |
| Patent titled: "Nucleic acid sequences from rice involved in seed development and uses thereof". Inventor; Tyagi AK, Kapoor S, Agarwal P; Patent File, 160/DEL/2007 |
|
|
|
|
| |
|
|
|
|
|
|
 |
|
